Basic design of the demonstration plant is developed according to the outputs of Action A1 which includes two units, Unit 1 to treatment greaves Category 2 and Unit 2 to recovery concentrated collagen from Unit 1 and wastewater Category 3 the final design is as follows:

Find out the initial design of LIFE Byprotval project

This action is devoted to every activities related to the develolpment of demonstration plant: 

 B1.1 Demonstration plant design

 B1.2 Construction and set-up of the demonstration plant Unit 1

→ B1.3 Construction of demonstration plant Unit 2 and set-up (in progress) 

                                                     Unit 1                                                                                                                   Unit 2

                                         Hydrolysis Reactor (5 m3)                                                                          Protein water concentrator (2m3)                

                                         LIFE byProtVal pilot plant is located in Almazán, Soria -Spain with 100 protein Tn/year capacity

 → B1.4 Process optimisation at the Demonstration Plant (in progress)

 B1.5 Input/output characterisation: properties analysed includes moisture, ash, fat and collagen content, elemental analysis, particle size, density. After this comparison total aminoacid profile was developed in order to evaluate the potential of the recovery protein hydrolysates for application as biostimulants being in glycine and in glutamic acid higher for category 3 wastewater. Both amino acids have the same behaviour for plant performance such as: improvement chlorophyll production, enhance plant growth, and nutrient uptake.  

During the process of transforming animal by-product into high value products is necesary to develop following actions:

 B2.1 Formulation of chemicals based on collagen derivatives (in progress)

 B2.2 Assessment of products suitability (in progress)

 Protein based product validation

In order to validate the bioestimulant solution from hydrolysed product of wastewater and PAP, in vitro germination bioassays by dosing different concentrations were developed:

Study of the growth of Chinese cabbage and lettuce seeds by dosing different concentrations of biostimulant solution. Control sample of Chinese cabbage (a). Growth of Chinese cabbage using a dosage of 0.15 (b). Lettuce growth using a dosage of 0.15 (c). 

Seeds growth by 19-42% with the optimal biostimulant dilution concentration in the range of 0.05-0.2%. ISO standard 16086-2 2012. 

 Validation of collagen hydrolysate as retanning agent

Leather obtained using protein based retanning agent

Three sub-actions have been planned to ensure the continuation of project results after the end of the project:

 B3.1 Market analysis 

      B3.2 Development of a business plan (in progress)

      B3.3 Development of a replicability and transferability plan (in progress)

Market dynamics for hydrolysate protein