Project evolution

A.1 Defined processes
A.2 Basic design of the demonstration plant
B.1 Construction and setting-up of the demonstration plant
B.2 Technology Validation
B.3 Development of a strategy to ensure sustainability, replicability and trasnferability
C.1 Monitoring and measurement of LIFE performace indicators
C.2 Environmental and socio-economic impact
D.1 Dissemination planning and execution

A.1 Defined Processes ✅

An enzymatic process is proposed for the extraction of collagen-based products from category 2 greaves and wastewater. This process has shown to be suitable for the treatment of non-tanned hides and skins and for PAPs. For this is needed following preparatory actions:

Captura_de_pantalla_2023-07-05_151416.png A1.1 selection of conditions for the extraction of collagen based products from greaves

 The proposed process consists of the following stages:


     Find out the defined processes in detail of LIFE Byprotval project

Captura_de_pantalla_2023-07-05_151416.png A1.2 Selection of the technologies for the extraction of collagen based produts from processing water.

 The proposed process consists of the following stages:



A.2 Basic prototype layout ✅

Basic design of the demonstration plant is developed according to the outputs of Action A1 which includes two units, Unit 1 to treatment greaves Category 2 and Unit 2 to recovery concentrated collagen from Unit 1 and wastewater Category 3 the final design is as follows:


Find out the initial design of LIFE Byprotval project

B.1 Demonstration plant for collagen derivatives extraction

This action is devoted to every activities related to the develolpment of demonstration plant: 

Captura_de_pantalla_2023-07-05_151416.png B1.1 Demonstration plant design

Captura_de_pantalla_2023-07-05_151416.png B1.2 Construction and set-up of the demonstration plant Unit 1

→ B1.3 Construction of demonstration plant Unit 2 and set-up (in progress

                                                     Unit 1                                                                                                                   Unit 2

                                         Hydrolysis Reactor (5 m3)                                                                          Protein water concentrator (2m3) Captura_de_pantalla_2023-07-05_135420.png               IMG20230126114623.jpg

                                         LIFE byProtVal pilot plant is located in Almazán, Soria -Spain with 100 protein Tn/year capacity

 → B1.4 Process optimisation at the Demonstration Plant (in progress)

Captura_de_pantalla_2023-07-05_151416.png B1.5 Input/output characterisation: properties analysed includes moisture, ash, fat and collagen content, elemental analysis, particle size, density. After this comparison total aminoacid profile was developed in order to evaluate the potential of the recovery protein hydrolysates for application as biostimulants being in glycine and in glutamic acid higher for category 3 wastewater. Both amino acids have the same behaviour for plant performance such as: improvement chlorophyll production, enhance plant growth, and nutrient uptake.  




B.2 Technology validation

During the process of transforming animal by-product into high value products is necesary to develop following actions:

 B2.1 Formulation of chemicals based on collagen derivatives (in progress)

 B2.2 Assessment of products suitability (in progress)

Captura_de_pantalla_2023-07-05_151416.png Protein based product validation

In order to validate the bioestimulant solution from hydrolysed product of wastewater and PAP, in vitro germination bioassays by dosing different concentrations were developed:Captura_de_pantalla_2023-07-05_153826.png

Study of the growth of Chinese cabbage and lettuce seeds by dosing different concentrations of biostimulant solution. Control sample of Chinese cabbage (a). Growth of Chinese cabbage using a dosage of 0.15 (b). Lettuce growth using a dosage of 0.15 (c). 

Seeds growth by 19-42% with the optimal biostimulant dilution concentration in the range of 0.05-0.2%. ISO standard 16086-2 2012. 

Captura_de_pantalla_2023-07-05_151416.png Validation of collagen hydrolysate as retanning agent


Leather obtained using protein based retanning agent

B.3 Development of a strategy to ensure sustainability, replicability and transferability

Three sub-actions have been planned to ensure the continuation of project results after the end of the project:

Captura_de_pantalla_2023-07-05_151416.png B3.1 Market analysis 

      B3.2 Development of a business plan (in progress)

      B3.3 Development of a replicability and transferability plan (in progress)


Market dynamics for hydrolysate protein

FaLang translation system by Faboba

This project has received funding from the LIFE Programme of the European Union under Grant Agreement LIFE16 ENV/ES/000467.

Information published in this website reflects only the authors’ view. CINEA and the European Commission cannot be held responsible for any use that may be made of the information contained therein.

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